GeneLancet presented at the 27th ASGCT meeting and TIDES-USA 2024

DOYLESTOWN, PA, June 11th, 2024-GeneLancet presented at the 27th ASGCT meeting and TIDES-USA 2024.

Our two presentations at the ASGCT meeting have received phenomenal responses. In our first presentation, titled “A Comparison Study Between Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Guide RNAs and the Discovery of LgRNA-33 as an Anti-HBV Agent with Multiple Mechanisms of Action,” we disclosed the superiority of LgRNA over sgRNA, the high-quality Lg-epegRNA for prime editing, and the discovery of highly potent LgRNA33 as a drug candidate for curing chronic HBV infection. In our second presentation, titled “Efficient and Precise Gene Editing by STAR (Seek-Tag-Amend-Release) Editing Enabled by the Synergistic Actions of a Cas9 Nickase, a segNA (STAR Editing Guide Nucleic Acids), and a DNA Dependent Polymerase”, we disclosed our exciting progress in design and optimization of segRNAs for precise gene editing by STAR editing approach and development of highly efficient segRNA nucleic acid constructs.
Our poster presentation at TIDES-USA 2024, titled “Next-generation Single Molecule Guide RNAs with Minimal Off-target-causing Impurities for Precise CRISPR Gene Editing,” disclosed GeneLancet’s innovative approach to solving the challenging issues in chemical productions of long CRISPR guide RNAs, including both sgRNA and pegRNA/epegRNA. These challenges include high production costs, extremely low yields (<6% for 100 nt sgRNA as reported by attending CDMOs; no data reported for pegRNA/epegRNA (125/~180 nt)), lack of robust analytical methods for quality control, the presence of inseparable truncated impurities that can cause catastrophic off-target effects, and low scalability (only gram scales till the present). We presented our LgRNA technologies that have dramatically improved the production efficiency, purity, and safety of these RNA molecules, paving the way for more precise and effective CRISPR gene editing.
We deeply value the feedback and effective scientific exchanges we’ve had with the esteemed audiences, as this plays a crucial role in shaping our continued research and its outcomes.