Ultra-Pure Ligated sgRNA (LgRNA) for Precise Gene Editing
GeneLancet Biosciences’ platform addresses key challenges in CRISPR gene editing. Today, CRISPR-Cas9 gene editing utilizes single guide RNA (sgRNA). While sgRNA is effective in achieving consistent editing results, synthetic sgRNA suffers from low overall product purity (<50% LC-MS purity), which results in reduced editing efficiency and specificity. Fully-functional, truncated, lot-dependent impurities in sgRNA pose catastrophic risks to patients.
The crRNA in our ligated single-molecule guide RNA (LgRNA) is 32 nt long, and its high purity is readily accessible by routine purifications before ligation, which selects the full-length product of tracrRNA. All GeneLancet’s LgRNA products contain undetectable truncated spacer sequences and are ultra-pure (up to 99% by LC/MS) with very low levels of biologically active impurities.